Journal: International Journal of Clinical and Experimental Pathology

Article Title: Neuritin 1 expression in human normal tissues and its association with various human cancers

doi:

Figure Lengend Snippet: Nrn1 mRNA expression in multiple tissues. Nrn1 cDNA (426 bp) was detected in the thymus, spleen, testicles, prostate, ovary, small intestine, colon and leukocytes (A), as well as the heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas (B). Glyceraldeyde 3-phosphate dehydrogenase (G3PDH) was used as a loading control. (C) Quantification of OD ratio for Nrn1 mRNA expression (n = 3). Each bar corresponds to mean ± Standard Deviation for individual tissues. (D) The clustering heat map analysis performed using R tools with the OD ratio of Nrn1 mRNA expression in each tissue.

Article Snippet: Endogenous peroxidase activity was blocked by incubation with 3% peroxide for 10 min followed by three washes in PBS for 5 min. Nonspecific binding was blocked by incubation with bovine serum albumin, and the sections were then incubated at 4°C overnight with polyclonal rabbit anti-Nrn1 (FL-142) antibody (cat. no. sc-25651; Santa Cruz Biotechnology, Santa Cruz, CA, USA) (1:50).

Techniques: Expressing, Control, Standard Deviation

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Neuritin 1 expression in human normal tissues and its association with various human cancers

doi:

Figure Lengend Snippet: Detection of Nrn1 in human tissues by immunohistochemistry. Areas of positive immunoreactivity are brown and indicated by arrows; nuclei are stained violet with hematoxylin. A. Hippocampus (positive control); B. Cerebellum; C. Cortex; D. Spinal cord; E. Alveolar epithelium; F. Colonic gland; G. Liver; H. Pancreatic epithelium; I. Negative control. Images are shown at 40 × magnification.

Article Snippet: Endogenous peroxidase activity was blocked by incubation with 3% peroxide for 10 min followed by three washes in PBS for 5 min. Nonspecific binding was blocked by incubation with bovine serum albumin, and the sections were then incubated at 4°C overnight with polyclonal rabbit anti-Nrn1 (FL-142) antibody (cat. no. sc-25651; Santa Cruz Biotechnology, Santa Cruz, CA, USA) (1:50).

Techniques: Immunohistochemistry, Staining, Positive Control, Negative Control

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Neuritin 1 expression in human normal tissues and its association with various human cancers

doi:

Figure Lengend Snippet: Subcellular localization of Nrn1 and its association with the lipid raft. A. SignalP results graphed by C-, S-, and Y-scores. Default (D-)cutoff was set to 0.5 (purple line). Genes with graphical intersections above the D-cutoff contain signal peptides. B. Transmembrane helices of Nrn1 predicted by TMHMM software. TMHMM plots are shown as probability (y-axis) of an amino acid (x-axis) residue sitting in a helix, inside, or outside summed over all possible model paths. The N-best prediction threshold was scored between 1 and 1.2 (purple line). Transmembrane, inside, and outside represent the probability of an amino acid being located within a transmembrane helix, on the cytoplasmic side of a membrane, and in the extracellular space, respectively. C. Nrn1 and caveolin 1 localization at the plasma membrane in SH-SY5Y cells. Scale bar = 50 μm. Detection of caveolin 1 and Nrn1 in SY5Y cell membrane protein extracts by Western blotting.

Article Snippet: Endogenous peroxidase activity was blocked by incubation with 3% peroxide for 10 min followed by three washes in PBS for 5 min. Nonspecific binding was blocked by incubation with bovine serum albumin, and the sections were then incubated at 4°C overnight with polyclonal rabbit anti-Nrn1 (FL-142) antibody (cat. no. sc-25651; Santa Cruz Biotechnology, Santa Cruz, CA, USA) (1:50).

Techniques: Software, Residue, Membrane, Clinical Proteomics, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Neuritin 1 expression in human normal tissues and its association with various human cancers

doi:

Figure Lengend Snippet: Expression level of Nrn1 in cancers including 31 kinds of cancers as indicated in the figure and paired normal tissues. T: tumor tissues, N: normal tissues, *P<0.05.

Article Snippet: Endogenous peroxidase activity was blocked by incubation with 3% peroxide for 10 min followed by three washes in PBS for 5 min. Nonspecific binding was blocked by incubation with bovine serum albumin, and the sections were then incubated at 4°C overnight with polyclonal rabbit anti-Nrn1 (FL-142) antibody (cat. no. sc-25651; Santa Cruz Biotechnology, Santa Cruz, CA, USA) (1:50).

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Neuritin 1 expression in human normal tissues and its association with various human cancers

doi:

Figure Lengend Snippet: A. Integrity of prognostic value of Nrn1 in BLCA; BRCA; CESC; COAD; GBM; KIHC; KIRP; LGG; READ; UCEC; LUA and OV patients. B. Prognostic value of Nrn1 in DLBC patients. HR: hazard ratio, CI: confidence interval, logRank P<0.01.

Article Snippet: Endogenous peroxidase activity was blocked by incubation with 3% peroxide for 10 min followed by three washes in PBS for 5 min. Nonspecific binding was blocked by incubation with bovine serum albumin, and the sections were then incubated at 4°C overnight with polyclonal rabbit anti-Nrn1 (FL-142) antibody (cat. no. sc-25651; Santa Cruz Biotechnology, Santa Cruz, CA, USA) (1:50).

Techniques: